β细胞自身免疫
Beta-cell autoimmunity.
作者信息
Fuchs Yannick F, Adler Kerstin, Bonifacio Ezio
机构信息
Preclinical Approaches to Stem Cell Therapy/Diabetes, Technische Universität Dresden, DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence, Dresden, Germany.
出版信息
Methods Mol Biol. 2012;933:265-74. doi: 10.1007/978-1-62703-068-7_17.
Beta cell destruction in autoimmune diabetes is accompanied by the presence of autoantibodies and autoreactive T cells against beta cell antigens. Autoantibodies to insulin are predictive of future diabetes in man and in the non-obese diabetic mouse model. Furthermore, the detection of peripheral autoreactive CD8(+) T cells in this mouse model is indicative of beta cell killing and correlates with the development of diabetes. We describe two protocols that are helpful for the detection of beta-cell autoimmunity in mice. The first protocol describes the detection of insulin-specific autoantibodies using a radio-binding assay. The other is a general CD8(+) T cell ELISpot protocol for the detection of peptide-specific responses of CD8(+) T cells from secondary lymphoid organs or pancreatic islets.
自身免疫性糖尿病中的β细胞破坏伴随着针对β细胞抗原的自身抗体和自身反应性T细胞的存在。胰岛素自身抗体可预测人类和非肥胖糖尿病小鼠模型未来是否会患糖尿病。此外，在该小鼠模型中检测外周自身反应性CD8(+) T细胞表明存在β细胞杀伤，并与糖尿病的发展相关。我们描述了两种有助于检测小鼠β细胞自身免疫的方案。第一种方案描述了使用放射结合试验检测胰岛素特异性自身抗体。另一种是通用的CD8(+) T细胞ELISpot方案，用于检测来自二级淋巴器官或胰岛的CD8(+) T细胞的肽特异性反应。
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