Dietrich Nadine, Hammes Hans-Peter
5th Medical Department, Universitätsmedizin Mannheim, University of Heidelberg, Mannheim, Germany.
Methods Mol Biol. 2012;933:291-302. doi: 10.1007/978-1-62703-068-7_19.
Retinal digestion is a commonly used method for studying experimental diabetic retinopathy in animal models. The method allows to assess qualitatively and quantitatively the morphology of the retinal vasculature, including characteristics of endothelial cells and pericytes. The digestion method uses the enzyme trypsin and enables the precise evaluation of venolar and arteriolar diameters, endothelial cell and pericyte numbers, and the formation of acellular capillaries.
视网膜消化是在动物模型中研究实验性糖尿病视网膜病变常用的方法。该方法能够定性和定量评估视网膜血管系统的形态，包括内皮细胞和周细胞的特征。消化方法使用胰蛋白酶，可精确评估小静脉和小动脉直径、内皮细胞和周细胞数量以及无细胞毛细血管的形成。
