Mathijs Iris, Houbracken Isabelle, Bouwens Luc
Cell Differentiation Lab, Vrije Universiteit Brussel, Brussels, Belgium.
Methods Mol Biol. 2012;933:317-22. doi: 10.1007/978-1-62703-068-7_21.
Genetic lineage tracing is an invaluable tool to demonstrate and measure neogenesis of beta cells from putative precursor cells. Cre-Lox recombination technology can be used for indelible labeling of a cohort of cells and following the fate of these cells and their progeny in animal models. Here, the combination is described of beta-galactosidase enzymatic staining with immunohistochemical staining to demonstrate labeled cells. This technique is performed in tissue cryosections.
遗传谱系追踪是一种用于证明和测量假定前体细胞中β细胞新生的宝贵工具。Cre-Lox重组技术可用于对一群细胞进行不可磨灭的标记，并追踪这些细胞及其后代在动物模型中的命运。本文描述了β-半乳糖苷酶酶促染色与免疫组织化学染色相结合以显示标记细胞的方法。该技术在组织冰冻切片中进行。
