Aitken A
Eur J Biochem. 1979 Nov 1;101(1):297-308. doi: 10.1111/j.1432-1033.1979.tb04243.x.
Cytochrome c-552 (soluble 'cytochrome f') from the unicellular cyanobacterium Synechococcus PCC 6312 (ATCC 27167) was purified and the primary structure determined. The proposed sequence consists of one polypeptide chain of 87 residues. The sequence was determined by a combination of chemical and enzymatic cleavage, manual and automatic sequencing and mass spectroscopy. This is the first amino acid sequence of this cytochrome from a unicellular cyanobacterium to be determined in a study of the variation in primary structure between phylogenetically distant cyanobacteria. The sequence is compared to the primary structures of the cytochrome from filamentous cyanobacteria and from eukaryotic algae. The significance of these sequence comparisons to the current hypotheses concerning the origin of eukaryotic cells and their chloroplasts is discussed.
对来自单细胞蓝细菌聚球藻属PCC 6312（ATCC 27167）的细胞色素c - 552（可溶性“细胞色素f”）进行了纯化，并确定了其一级结构。所提出的序列由一条87个残基的多肽链组成。该序列通过化学和酶切、手动和自动测序以及质谱分析相结合的方法确定。这是在对系统发育上距离较远的蓝细菌之间一级结构变异的研究中首次确定的来自单细胞蓝细菌的这种细胞色素的氨基酸序列。将该序列与丝状蓝细菌和真核藻类的细胞色素一级结构进行了比较。讨论了这些序列比较对于当前有关真核细胞及其叶绿体起源假说的意义。
