Stellwagen N C, Holmes D L
Department of Biochemistry, University of Iowa, Iowa City 52242.
Electrophoresis. 1990 Aug;11(8):649-52. doi: 10.1002/elps.1150110813.
A paradox was observed in a previous study of the electrophoresis of linear DNA fragments in agarose gels (D. L. Holmes and N. C. Stellwagen, Electrophoresis 1990, 11, 5-15). The pore size of the agarose matrix was more accurately determined if the root-mean-square radius of gyration was used to measure DNA macromolecular size. However, the Ogston equations were obeyed and other gel parameters such as the apparent fiber radius and fiber volume appeared to be better described if the geometric mean radius was used to measure DNA size. This paradox can be resolved if relative mobilities (with respect to the smallest DNA molecule in the data set) are used to construct the Ferguson plots, instead of absolute mobilities. Using relative mobilities and the root-mean-square radius of gyration, the Ogston equations are obeyed and the pore size of the matrix is consistent with values determined by other methods.
在先前一项关于线性DNA片段在琼脂糖凝胶中电泳的研究中观察到一个悖论（D. L. 霍姆斯和N. C. 斯特尔wagen，《电泳》，1990年，第11卷，5 - 15页）。如果使用均方根回转半径来测量DNA大分子大小，琼脂糖基质的孔径能更准确地确定。然而，如果使用几何平均半径来测量DNA大小，则奥格斯顿方程成立，并且其他凝胶参数（如表观纤维半径和纤维体积）似乎能得到更好的描述。如果使用相对迁移率（相对于数据集中最小的DNA分子）来构建弗格森图，而不是绝对迁移率，那么这个悖论就能得到解决。使用相对迁移率和均方根回转半径，奥格斯顿方程成立，并且基质的孔径与通过其他方法确定的值一致。
