Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
J Appl Genet. 2012 Nov;53(4):423-33. doi: 10.1007/s13353-012-0108-y. Epub 2012 Aug 16.
In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.
在这项研究中，我们调查了定位在 Y 染色体上的重复序列。重复 DNA 序列代表了真核生物基因组的重要部分，其中很大一部分包括串联重复的序列。由于使用了用于 Y 染色体分离的激光显微切割技术，随后进行聚合酶链反应（PCR）、克隆和序列分析，因此可以有效地快速分离重复单元。我们将衍生的重复单元应用于牛科的九个部落的成员中。除了我们使用已知重复序列的 Bos taurus 和 Bubalus bubalis 的 Y 染色体之外，衍生的序列还被用作荧光原位杂交的探针，用于牛科的九个部落的成员。我们调查了重复单元在部落中的分布及其在 Y 染色体上的定位。序列变异的共享将表明共同的起源，而快速的水平进化应该能够区分密切相关的物种或亚种。
