长期暴露于铝的幼兔大脑中mRNA的可翻译性。

Translatability of mRNA from brain of infant rabbits exposed chronically to aluminium.

作者信息

Nicholls D M, Speares G M, Asina S, Miller A C

机构信息

Department of Biology, York University, Ontario, Canada.

出版信息

Int J Biochem. 1990;22(10):1119-25. doi: 10.1016/0020-711x(90)90109-g.

DOI:10.1016/0020-711x(90)90109-g

Abstract

Polysomes were isolated from the brain of infant rabbits at 22 days of age. The animals received s.c. injections 3 times weekly of aluminium (Al) maltolate (3 mg Al/kg body wt) or Al lactate (16 mg Al/kg body wt) from 5 days of age. 2. The polysomes were used to direct the incorporation of [14C]leucine into peptides in a brain protein synthesizing system and exhibited a decreased activity when obtained from aluminum exposed infants. 3. The mRNA obtained from the polysomes was used to direct the incorporation of [35S]methionine into peptides in an mRNA dependent rabbit reticulocyte lysate. The translatability of the mRNA derived from aluminum exposed infant brains was significantly lower than that of preparations from control infant rabbits. 4. Al bound to maltolate, a ligand soluble in lipids as well as water, was considerably more detrimental to brain protein synthesis than Al bound to lactate.

摘要

从22日龄幼兔的大脑中分离出多核糖体。这些动物从5日龄开始每周皮下注射3次苹果酸铝（3毫克铝/千克体重）或乳酸铝（16毫克铝/千克体重）。
多核糖体被用于在脑蛋白合成系统中指导[14C]亮氨酸掺入肽中，并且当从暴露于铝的幼兔中获得时，其活性降低。
从多核糖体获得的mRNA被用于在依赖mRNA的兔网织红细胞裂解物中指导[35S]甲硫氨酸掺入肽中。来自暴露于铝的幼兔大脑的mRNA的可翻译性明显低于来自对照幼兔制剂的可翻译性。
与苹果酸结合的铝（苹果酸是一种既溶于脂质又溶于水的配体）对脑蛋白合成的损害比与乳酸结合的铝大得多。