远端小肠切除对大鼠小肠中ACAT活性及微粒体脂质成分的影响。

Effect of distal small bowel resection on ACAT activity and microsomal lipid composition in rat small intestine.

Molina M T, Vazquez C M, Ruiz-Gutierrez V

Departamento Fisiología y Biología Animal, Facultad de Farmacia, Sevilla, España.

Int J Biochem. 1990;22(10):1153-7. doi: 10.1016/0020-711x(90)90114-i.

Abstract

The acyl-CoA:cholesterol acyltransferase (ACAT) activity and lipid composition of intestinal microsomal membrane were investigated 6 weeks after both 50 and 75% distal small bowel resection (DSBR). 2. No changes in both microsomal ACAT activity and cholesteryl ester levels were found, while microsomal non-esterified cholesterol content was increased after the surgical operation. 3. The total phospholipid content of the microsomes did not change as a result of DSBR. 4. The microsomal phospholipid fatty acid composition showed a significant increase in saturated fatty acids together with no changes in both total monounsaturated and total polyunsaturated fatty acids after resection. 5. An increase in the levels of linoleic acid accompanied by a decrease in arachidonic acid was found in remnant intestine of resected rats.

摘要

在进行50%和75%远端小肠切除（DSBR）6周后，研究了肠微粒体膜的酰基辅酶A：胆固醇酰基转移酶（ACAT）活性和脂质组成。2. 未发现微粒体ACAT活性和胆固醇酯水平有变化，而手术后微粒体非酯化胆固醇含量增加。3. DSBR并未导致微粒体总磷脂含量发生变化。4. 微粒体磷脂脂肪酸组成显示，切除术后饱和脂肪酸显著增加，而总单不饱和脂肪酸和总多不饱和脂肪酸均无变化。5. 在切除大鼠的残余肠中发现亚油酸水平升高，同时花生四烯酸水平降低。