Vázquez C M, Muriana F J, Ruiz-Gutierrez V
Departamento de Fisiología y Biología Animal, Facultad de Farmacia, Sevilla, Spain.
Lipids. 1993 May;28(5):471-3. doi: 10.1007/BF02535948.
We have studied the activities of delta 9, delta 6 and delta 5 desaturases in rat liver and jejunum when the entero-hepatic circulation was interrupted by either 50 or 75% intestinal resection. Desaturase activities in caecal mucosa were also determined in control and operated rats. Distal small bowel resection significantly altered the activities of desaturase enzymes in liver, jejunum and caecum. Thus, the delta 9 desaturase activity was lower in hepatic microsomes from operated animals, and this decrease was not related to the extent by which the intestine was removed. However, a significant increase in both delta 6 and delta 5 desaturases was found in these animals compared to sham-operated rats, the increase in both desaturases being higher after 75% than after 50% intestinal resection. The activities of delta 9 and delta 5 desaturases were significantly increased in jejunal mucosa of resected rats. The activity of delta 6 desaturase was increased only in 50% resected-animals. An increase in delta 6 desaturase activity was observed in caecal mucosa after resection, together with a decrease in delta 9 desaturase and no change in delta 5 desaturase activities. Enzyme activities were highest in the liver relative to the activity in jejunal and caecal homogenates. In sham rats, the caecal mucosa showed higher delta 9 and delta 6 but lower delta 5 desaturase activities than in jejunal mucosa.
我们研究了在肠肝循环被50%或75%肠切除中断时，大鼠肝脏和空肠中δ9、δ6和δ5去饱和酶的活性。还测定了对照大鼠和手术大鼠盲肠黏膜中的去饱和酶活性。远端小肠切除显著改变了肝脏、空肠和盲肠中去饱和酶的活性。因此，手术动物肝脏微粒体中的δ9去饱和酶活性较低，且这种降低与肠切除的程度无关。然而，与假手术大鼠相比，这些动物的δ6和δ5去饱和酶均显著增加，75%肠切除后两种去饱和酶的增加高于50%肠切除后。切除大鼠空肠黏膜中δ9和δ5去饱和酶的活性显著增加。δ6去饱和酶的活性仅在50%切除的动物中增加。切除后盲肠黏膜中观察到δ6去饱和酶活性增加，同时δ9去饱和酶活性降低，δ5去饱和酶活性无变化。相对于空肠和盲肠匀浆中的活性，肝脏中的酶活性最高。在假手术大鼠中，盲肠黏膜的δ9和δ6去饱和酶活性高于空肠黏膜，但δ5去饱和酶活性低于空肠黏膜。
