Department of Animal Science and Interdepartmental Graduate Program in Nutritional Sciences, Iowa State University, Ames, Iowa 50011, USA.
J Anim Sci. 2012 Aug;90(8):2692-8. doi: 10.2527/jas.2012-4482.
To examine the effects of cattle breed on the clearance rate of an injectable mineral product, 10 Angus and 10 Simmental steers were blocked by breed and initial BW (332 ± 33 kg) and injected with either Multimin 90 (MM) or sterilized saline (CON) at a dose of 1 mL/45 kg BW. Multimin 90 contains 15 mg Cu/mL (as Cu disodium EDTA), 60 mg Zn/mL (as Zn disodium EDTA), 10 mg Mn/mL (as Mn disodium EDTA), and 5 mg Se/mL (as sodium selenite). Steers received a corn-silage-based diet, and inorganic sources of Cu, Zn, Mn, and Se were supplemented at NRC recommended amounts. Jugular blood was collected immediately before injection and at 8 and 10 h post-injection and on days 1, 8, and 15 post-injection. Liver biopsies were collected 3 d before injection and on days 1, 8, and 15 post-injection. Liver and plasma mineral concentration and glutathione peroxidase (GSH-Px) activity data were analyzed as repeated measures. Plasma concentrations of Zn, Mn, and Se were greater (P = 0.01) and Cu tended to be greater (P = 0.12) post-injection in MM steers compared with the CON steers. Regardless of treatment, Simmental cattle had lower plasma concentrations of Cu, Zn, and Se (P ≤ 0.05) when compared with Angus cattle. Erythrocyte GSH-Px activity was greater (P = 0.01) in MM steers compared with CON steers. Liver concentrations of Cu, Zn, and Se were greater (P = 0.05) in MM steers compared with CON steers post-injection. Liver Mn concentrations tended to be greater (P = 0.06) in MM steers compared with CON steers in the days post-injection. Interestingly, Simmental cattle exhibited greater (P = 0.01) liver Mn concentrations in the days after injection compared with Angus cattle (7.0 and 6.0 mg Mn/kg for Simmental and Angus cattle, respectively), regardless of treatment. It is unclear if this breed difference is biologically relevant; however, these data may suggest that differences in liver excretion of Mn exist between the two breeds. Overall, use of an injectable trace mineral increased liver concentrations of Cu and Se through the 15-d sampling period, suggesting that this injectable mineral is an adequate way to improve Cu and Se status of cattle through at least 15 d.
为了研究牛品种对可注射矿物质清除率的影响，将 10 头安格斯牛和 10 头西门塔尔牛按品种和初始体重（332±33kg）分组，然后分别以 1ml/45kgBW 的剂量注射 Multimin90（MM）或无菌生理盐水（CON）。Multimin90 含有 15mgCu/ml（以 EDTA 二钠铜形式存在）、60mgZn/ml（以 EDTA 二钠锌形式存在）、10mgMn/ml（以 EDTA 二钠锰形式存在）和 5mgSe/ml（以亚硒酸钠形式存在）。牛只接受以玉米青贮为基础的日粮，铜、锌、锰和硒的无机来源按 NRC 推荐量补充。在注射前即刻和注射后 8 小时和 10 小时以及注射后 1 天、8 天和 15 天采集颈静脉血。在注射前 3 天和注射后 1 天、8 天和 15 天采集肝活检。肝脏和血浆矿物质浓度和谷胱甘肽过氧化物酶（GSH-Px）活性数据作为重复测量进行分析。与 CON 牛相比，MM 牛在注射后（P=0.01）锌、锰和硒的血浆浓度更高（P=0.01），且铜的浓度也有升高的趋势（P=0.12）。无论治疗如何，西门塔尔牛的铜、锌和硒的血浆浓度（P≤0.05）均低于安格斯牛。与 CON 牛相比，MM 牛的红细胞 GSH-Px 活性更高（P=0.01）。与 CON 牛相比，MM 牛在注射后（P=0.05）肝脏中铜、锌和硒的浓度更高。与 CON 牛相比，MM 牛在注射后几天内肝脏中的锰浓度有升高的趋势（P=0.06）。有趣的是，无论治疗如何，西门塔尔牛在注射后几天内（分别为 7.0 和 6.0mgMn/kg）的肝脏锰浓度均高于安格斯牛（P=0.01）。这种品种差异是否具有生物学意义尚不清楚；然而，这些数据表明，这两个品种之间可能存在肝脏排泄锰的差异。总的来说，使用可注射微量元素增加了牛肝脏中铜和硒的浓度，在 15 天的采样期内，这表明这种可注射矿物质是通过至少 15 天来改善牛铜和硒状态的一种有效方法。
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