Laboratorio de Inmunología y Parasitología Molecular, IBSAL-CIETUS, Universidad de Salamanca, Salamanca, Spain.
Parasite Immunol. 2012 Dec;34(12):570-80. doi: 10.1111/pim.12004.
Despite progress in understanding the role of nitric oxide (NO) in the pathogenesis of helminth infections, the role in strongyloidosis is unknown. Firstly, we studied the production of NO in mice infected with Strongyloides venezuelensis as well as in macrophage cultures stimulated with parasite antigens. Somatic larvae 3 (L3) and excretory-secretory female antigens stimulate specific NO production measured by Griess reaction and expression of inducible NO synthase by RT-PCR and quantitative PCR. Moreover, mice infected with S. venezuelensis produce NO in migration stages. Secondly, we analysed the effect of NO production on L3 and females of S. venezuelensis using NO donors such as diethylenetriamine and 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene. Parasites died after NO donor treatment in a dose-dependent manner. Finally, apoptotic mechanisms are involved in the death of S. venezuelensis larvae.
尽管在理解一氧化氮（NO）在寄生虫感染发病机制中的作用方面取得了进展，但在旋毛虫病中的作用尚不清楚。首先，我们研究了感染委内瑞拉旋毛虫的小鼠以及用寄生虫抗原刺激的巨噬细胞培养物中 NO 的产生。体幼虫 3（L3）和排泄-分泌雌性抗原刺激通过格里斯反应测量的特异性 NO 产生以及通过 RT-PCR 和定量 PCR 表达诱导型 NO 合酶。此外，感染委内瑞拉旋毛虫的小鼠在迁移阶段产生 NO。其次，我们使用一氧化氮供体（如二亚乙基三胺和 3,3-双（氨基乙基）-1-羟基-2-氧代-1-三嗪）分析 NO 产生对委内瑞拉旋毛虫 L3 和雌性的影响。寄生虫在一氧化氮供体处理后以剂量依赖性方式死亡。最后，旋毛虫幼虫的死亡涉及凋亡机制。
