Catholic University of Leuven, Department of Periodontology, Leuven, Belgium.
J Periodontol. 2013 Jun;84(6):801-11. doi: 10.1902/jop.2012.120261. Epub 2012 Aug 16.
The aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria.
Subgingival plaque samples from 35 patients (healthy and periodontitis patients) were analyzed. Growth inhibition of the periodontal pathogens Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans was examined using the agar overlay technique and agar well diffusion method. The quantification of the inhibitory effect was checked with the agar well diffusion method.
Using the agar overlay technique, the prevalence of strains antagonistic toward P. gingivalis, A. actinomycetemcomitans, and F. nucleatum was found to be higher in healthy individuals than in individuals with periodontitis, but this could not be validated by the agar well diffusion assay. Compared with the antagonistic activity of the isolated strains, the probiotic strains overall showed a stronger inhibition of the periodontal pathogens.
It was shown that some oral bacteria can cause antagonism toward periodontopathogens, and these observations underline the therapeutic potential of applications that stimulate oral health by the application of beneficial effector strains.
本研究旨在比较健康个体和患病个体中有益共生菌的流行情况，并研究一些商业膳食益生菌对牙周致病菌的抑制作用，同时将其与口腔来源的有益细菌的抑制效果进行比较。
分析了 35 名患者（健康和牙周炎患者）的龈下菌斑样本。使用琼脂覆盖技术和琼脂孔扩散法检测牙周病病原体牙龈卟啉单胞菌、中间普氏菌、核梭杆菌和伴放线放线杆菌的生长抑制作用。用琼脂孔扩散法检查抑制效果的定量。
使用琼脂覆盖技术，发现健康个体中拮抗牙龈卟啉单胞菌、伴放线放线杆菌和核梭杆菌的菌株流行率高于牙周炎患者，但琼脂孔扩散法无法验证这一结果。与分离菌株的拮抗活性相比，益生菌菌株总体上对牙周致病菌表现出更强的抑制作用。
研究表明，一些口腔细菌可以对牙周致病菌产生拮抗作用，这些观察结果强调了通过应用有益效应菌株来刺激口腔健康的应用的治疗潜力。
