Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029, China.
Molecules. 2012 Aug 16;17(8):9855-67. doi: 10.3390/molecules17089855.
The object of this study was to identify the major active ingredients of the Chinese Traditional Medicine Jiang-Zhi-Ning (JZN) based on the high performance liquid chromatography (HPLC) profiles of plasma samples obtained from beagle dogs at different times after intragastric administration of JZN, crude JZN extracts, different extracted fractions, different subfractions of the active fraction and different isolated ingredients. 2-Hydroxy-1-methoxyaporphin (2H1M), an alkaloid from Nelumbo nucifera, one of the herbs that make up JZN, was identified as the constituent showing the major pharmacodynamic effect. The major metabolites of 2H1M were analyzed and identified as N-demethyl-2-hydroxy-1-methoxyaporphine-2-O-glycuronic acid, 2-hydroxy-1-methoxy-aporphine-2-O-glycuronic acid and 2-hydroxy-1-methoxy-aporphine-2-O-sulphate. This study provided a comprehensive insight into the active components of JZN.
本研究的目的是基于灌胃给予犬后不同时间的血浆样品的高效液相色谱（HPLC）图谱，鉴定中药降脂宁（JZN）的主要活性成分，JZN 粗提物、不同提取部分、活性部分的不同亚部分和不同分离成分。2-羟基-1-甲氧基阿朴啡（2H1M），JZN 组成草药之一的荷叶中的一种生物碱，被鉴定为具有主要药效作用的成分。分析并鉴定了 2H1M 的主要代谢物为 N-去甲基-2-羟基-1-甲氧基阿朴啡-2-O-葡萄糖醛酸、2-羟基-1-甲氧基阿朴啡-2-O-葡萄糖醛酸和 2-羟基-1-甲氧基阿朴啡-2-O-硫酸盐。本研究全面了解了 JZN 的活性成分。
