软膏剂中丁酸氯倍他松的液相色谱测定法
Liquid chromatographic determination of clobetasone-17-butyrate in ointments.
作者信息
Patel A G, Patel R B, Patel M R
机构信息
Cadila Laboratories Ltd, Analytical Research Laboratory, Gujarat, India.
出版信息
J Assoc Off Anal Chem. 1990 Nov-Dec;73(6):893-5.
A liquid chromatographic (LC) method has been developed for determination of clobetasone-17-butyrate in ointment using clobetasone propionate as an internal standard. Separation was carried out on a C18 reverse-phase column using water-methanol as a mobile phase. Methylparaben and propylparaben (both sodium salt) used as preservatives did not interfere with separation. Compounds are detected photometrically at 235 nm. Mean assay results for 0.05% commercial ointments were 100.36% (n = 5). Mean recovery of clobetasone-17-butyrate added to commercial ointment was 99.89%.
已开发出一种液相色谱（LC）方法，以丙酸氯倍他索为内标物，用于测定软膏剂中丁酸氯倍他松的含量。采用水 - 甲醇作为流动相，在C18反相柱上进行分离。用作防腐剂的对羟基苯甲酸甲酯和对羟基苯甲酸丙酯（均为钠盐）不干扰分离。在235nm处通过光度法检测化合物。0.05%商业软膏剂的平均含量测定结果为100.36%（n = 5）。添加到商业软膏剂中的丁酸氯倍他松的平均回收率为99.89%。
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