Salvador Ricardo, Ferrelli M Leticia, Berretta Marcelo F, Mitsuhashi Wataru, Biedma Marina E, Romanowski Víctor, Sciocco-Cap Alicia
Instituto de Microbiología y Zoología Agrícola, Castelar, Buenos Aires, Argentina.
Virus Genes. 2012 Dec;45(3):610-3. doi: 10.1007/s11262-012-0800-3. Epub 2012 Aug 17.
The Epinotia aporema Granulovirus GP37 protein gene has been identified, located, and sequenced. This gene was similar to other baculovirus gp37, to entomopoxvirus fusolin gene, and to the chitin-binding protein gene of bacteria. Sequence analysis indicated that the open reading frame is 669 bp long (the smallest gp37 sequenced at present) and encodes a predicted 222-amino acid protein. This protein is glycosylated and specifically recognized by an entomopoxvirus fusolin antiserum. The pairwise comparison of EpapGV gp37 gene product with all the baculovirus sequences in GenBank yields high similarity values ranging from 45 to 63 % with Cydia pomonella Granulovirus gp37 being the most closely related. The phylogenetic analysis interestingly grouped the granuloviruses in a cluster more closely related to entomopoxviruses than to nucleopolyhedroviruses, suggesting a possible horizontal transfer event between the granulovirus group and the entomopoxvirus group.
苹果小卷蛾颗粒体病毒GP37蛋白基因已被鉴定、定位和测序。该基因与其他杆状病毒gp37、昆虫痘病毒fusolin基因以及细菌的几丁质结合蛋白基因相似。序列分析表明，开放阅读框长669bp（目前测序的最小gp37），编码一个预测的222个氨基酸的蛋白质。该蛋白质被糖基化，并被昆虫痘病毒fusolin抗血清特异性识别。将苹果小卷蛾颗粒体病毒gp37基因产物与GenBank中所有杆状病毒序列进行成对比较，相似度值较高，范围为45%至63%，其中苹果蠹蛾颗粒体病毒gp37关系最为密切。系统发育分析有趣地将颗粒体病毒归为一个与昆虫痘病毒比与核型多角体病毒关系更密切的簇，这表明颗粒体病毒组和昆虫痘病毒组之间可能存在水平转移事件。
