Jiang Hui, Shi Chunhua, Xie Yongshu, Xu Xiaolong, Liu Qingliang
Department of Chemistry, University of Science and Technology of China, Hefei, 230026, P. R. China.
Indian J Biochem Biophys. 2003 Oct;40(5):350-3.
The effect of sodium dodecyl sulfate (SDS) on purified tobacco leaf PPO (PPO II) was investigated at various pHs and temperatures. SDS increased the activity of PPO II due to the formation of SDS-PPO II complex, leading to conformational changes, thus making access to active center easier. The relationship between the activity and the molar ratio of SDS-PPO II to PPO II showed that the critical point reached a plateau of activity at the molar ratio of about 1.2. The pH had a significant effect on interaction between SDS and PPO II, as compared to PPO II. The optimum catalytic temperature of the complex rose by 10 degrees C, suggesting that stabilization of the structure had been improved by the formation of complex.
研究了在不同pH值和温度下，十二烷基硫酸钠（SDS）对纯化的烟草叶片多酚氧化酶（PPO II）的影响。由于形成了SDS-PPO II复合物，SDS提高了PPO II的活性，导致构象变化，从而使活性中心更容易接近。SDS-PPO II与PPO II的活性与摩尔比之间的关系表明，在摩尔比约为1.2时达到活性平台的临界点。与PPO II相比，pH值对SDS与PPO II之间的相互作用有显著影响。复合物的最佳催化温度升高了10摄氏度，表明复合物的形成改善了结构的稳定性。
