Huang Wei-Yi, Chen Dong-Hui, Ning Li, Wang Li-Wei
Department of Oncology, Affiliated People's 1st Hospital, Shanghai Jiaotong University, Shanghai, China.
Asian Pac J Cancer Prev. 2012;13(5):1823-7. doi: 10.7314/apjcp.2012.13.5.1823.
The gene encoding the Nin one binding (NOB1) protein which plays an essential role in protein degradation has been investigated for possible tumor promoting functions. The present study was focused on NOB1 as a possible therapeutic target for breast cancer treatment. Lentivirus mediated NOB1 siRNA transfection was used to silence the NOB1 gene in two established breast cancer cell lines, MCF-7 and MDA-MB-231, successful transfection being confirmed by fluorescence imaging. NOB1 deletion caused significant decline in cell proliferation was observed in both cell lines as investigated by MTT assay. Furthermore the number and size of the colonies formed were also significantly reduced in the absence of NOB1. Moreover NOB1 gene knockdown arrested the cell cycle and inhibited cell cycle related protein expression. Collectively these results indicate that NOB1 plays an essential role in breast cancer cell proliferation and its gene expression could be a therapeutic target.
编码在蛋白质降解中起关键作用的Nin1结合（NOB1）蛋白的基因，已被研究其可能的肿瘤促进功能。本研究聚焦于NOB1作为乳腺癌治疗的潜在治疗靶点。采用慢病毒介导的NOB1 siRNA转染，使两种已建立的乳腺癌细胞系MCF-7和MDA-MB-231中的NOB1基因沉默，通过荧光成像确认成功转染。通过MTT法检测发现，在这两种细胞系中，NOB1缺失均导致细胞增殖显著下降。此外，在没有NOB1的情况下，形成的集落数量和大小也显著减少。而且，NOB1基因敲低使细胞周期停滞并抑制细胞周期相关蛋白表达。这些结果共同表明，NOB1在乳腺癌细胞增殖中起关键作用，其基因表达可能成为治疗靶点。
