Guo Jian-Xin, Tao Qing-Song, Lou Peng-Rong, Chen Xiao-Chun, Chen Jun, Yuan Guang-Bo
Cancer Chemotherapy Center, The First People Hospital of Ningbo, Ningbo, Zhejiang, China.
Asian Pac J Cancer Prev. 2012;13(5):2263-7. doi: 10.7314/apjcp.2012.13.5.2263.
MicroRNAs (miRNAs) play important roles in carcinogenesis. The aim of the present study was to explore the effects of miR-181b on gastric cancer.
The expression level of miR-181b was quantified by qRT-PCR. MTT, flow cytometry and matrigel invasion assays were used to test proliferation, apoptosis and invasion of miR-181b stable transfected gastric cancer cells.
miR-181b was aberrantly overexpressed in gastric cancer cells and primary gastric cancer tissues. Further experiments demonstrated inducible expression of miR-181b by Helicobacter pylori treatment. Cell proliferation, migration and invasion in the gastric cancer cells were significantly increased after miR-181b transfection and apoptotic cells were also increased. Furthermore, overexpression of miR-181b downregulated the protein level of tissue inhibitor of metalloproteinase 3 (TIMP3).
The upregulation of miR-181b may play an important role in the progress of gastric cancer and miR-181b maybe a potential molecular target for anticancer therapeutics of gastric cancer.
微小RNA（miRNA）在肿瘤发生过程中发挥重要作用。本研究旨在探讨miR-181b对胃癌的影响。
采用qRT-PCR定量检测miR-181b的表达水平。运用MTT、流式细胞术和基质胶侵袭实验检测miR-181b稳定转染的胃癌细胞的增殖、凋亡及侵袭能力。
miR-181b在胃癌细胞和原发性胃癌组织中异常高表达。进一步实验表明，幽门螺杆菌处理可诱导miR-181b表达。miR-181b转染后，胃癌细胞的增殖、迁移和侵袭能力显著增强，凋亡细胞也增多。此外，miR-181b过表达下调了金属蛋白酶组织抑制剂3（TIMP3）的蛋白水平。
miR-181b的上调可能在胃癌进展中起重要作用，miR-181b可能是胃癌抗癌治疗的潜在分子靶点。
