Department of Chemistry, Faculty of Science and Literature, Süleyman Demirel University, 32260 Isparta, Turkey.
J Pharm Biomed Anal. 2012 Dec;71:139-43. doi: 10.1016/j.jpba.2012.06.023. Epub 2012 Jun 26.
In this work, dissociation constants values of seven beta lactam antibiotics in water and acetonitrile-water mixtures using spectrophotometric and reversed phase liquid chromatography methods were determined. The dissociation constant values of these compounds were calculated by NLREG and STAR programs. Aqueous pK(a) values of beta lactam antibiotics were calculated with extrapolation by means of the Yasuda-Shedlovsky and mole fraction equations. Finally, application of the different techniques was compared to the determination of aqueous pK(a) values of investigated compounds.
在这项工作中，使用分光光度法和反相液相色谱法测定了七种β-内酰胺抗生素在水中和乙腈-水混合物中的离解常数值。这些化合物的离解常数值通过 NLREG 和 STAR 程序计算。通过 Yasuda-Shedlovsky 和摩尔分数方程的外推法计算了β-内酰胺抗生素的水相 pK(a)值。最后，比较了不同技术在测定所研究化合物的水相 pK(a)值方面的应用。
