Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia.
Eur J Obstet Gynecol Reprod Biol. 2012 Dec;165(2):194-8. doi: 10.1016/j.ejogrb.2012.08.003. Epub 2012 Aug 16.
The aim of this study was to determine the expression of the anaphylatoxin receptors complement C3a receptor (C3aR) and C5a receptor (C5aR) in the placentas of pregnancies complicated by severe early onset preeclampsia.
We recruited women with pregnancies complicated by severe early-onset preeclampsia (n=19, 11 of which were further complicated with IUGR) and women with preterm pregnancies not affected by preeclampsia (n=8). Gene and protein expression of C3aR and C5aR was analysed by quantitative RT-PCR and Western blotting, respectively.
C3aR was detected in the Hofbauer cells in the villous stroma of the placenta. C5aR staining was detected in the syncytiotrophoblast and endothelial cells. We found significantly decreased expression of C3aR mRNA and protein expression in placentas with preeclampsia compared to controls. However, C5aR expression was not significantly different between preeclamptic and control placentas at either the mRNA or protein level.
Decreased C3aR expression indicates a dysregulation of the complement system in the placentas of preeclamptic women. Further studies would elucidate the exact mechanisms that complement has in preeclampsia.
本研究旨在确定过敏毒素受体补体 C3a 受体 (C3aR) 和 C5a 受体 (C5aR) 在重度早发型子痫前期合并胎盘中的表达。
我们招募了患有重度早发型子痫前期（n=19，其中 11 例进一步并发 IUGR）和未受子痫前期影响的早产孕妇（n=8）的孕妇。通过定量 RT-PCR 和 Western blot 分别分析 C3aR 和 C5aR 的基因和蛋白表达。
在胎盘绒毛间质的 Hofbauer 细胞中检测到 C3aR。C5aR 染色在合体滋养层细胞和内皮细胞中检测到。与对照组相比，我们发现子痫前期胎盘中 C3aR mRNA 和蛋白表达显著降低。然而，在 mRNA 或蛋白水平，子痫前期胎盘与对照组的 C5aR 表达无显著差异。
C3aR 表达降低表明子痫前期妇女胎盘补体系统失调。进一步的研究将阐明补体在子痫前期中的确切作用机制。
