Klebanovskaia I A, Starostina O Iu, Ballad N E, Ivashchenko E A
Med Parazitol (Mosk). 1990 Nov-Dec(6):45-7.
An indirect method for the determination of antibodies to Opisthorchis antigens has been used in the elaboration of enzyme immunoassay for opisthorchiasis diagnosis. A visual method for the assessment of the results has been suggested. Its diagnostic efficacy has been determined. With the study of 1722 sera of the control subjects, the test specificity was 97.5%. Test sensitivity in the acute stage of the disease (40 patients) was about 100%, in the chronic stage (435 patients) it depended on oocyst production by the parasite. With less than 10 oocysts per I g feces, test sensitivity did not exceed 34.3%, with more than 1000 oocysts per I g feces it reached 94.4%. Mass population screenings, using the test in question, in 2 opisthorchiasis foci with different levels of endemia have shown that their results correspond to the data of copro-ovoscopy. The use of the test is most promising for the diagnosis of early phase of opisthorchiasis and the assessment of epidemiological situation in the foci.
一种间接测定抗华支睾吸虫抗原抗体的方法已用于研制华支睾吸虫病诊断的酶免疫测定。已提出一种评估结果的直观方法，并确定了其诊断效能。在对1722份对照受试者血清进行研究时，该检测的特异性为97.5%。该检测在疾病急性期（40例患者）的敏感性约为100%，在慢性期（435例患者）则取决于寄生虫的卵囊产生情况。每克粪便中卵囊少于10个时，检测敏感性不超过34.3%，每克粪便中卵囊多于1000个时，敏感性达到94.4%。在两个不同流行程度的华支睾吸虫病疫源地使用该检测进行大规模人群筛查表明，其结果与粪便虫卵检查的数据相符。该检测对于华支睾吸虫病早期诊断及疫源地流行病学情况评估最具前景。
