Dou Bo, Wang Jiangyong, Zhang Yingze, Tian Dehu, Bai Jiangbo, Feng Jianshu, Ni Huawei
Department of Orthopaedics, Lulong Hospital, Lulong Hebei, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 Jul;26(7):869-73.
Triptolide can suppress immunological rejection reaction. To investigate the effect of triptolide on allogenic tendon transplantation in repairing tendon defect in chicken.
The defect model of the third toes tendon was established in 64 healthy-cleaning male Leghorn chickens (4-month-old, weighing 1.9-2.3 kg), which underwent allogenic tendon transplantation for repairing and were divided into 2 groups randomly (n=32). Triptolide feeding [100 microg/(kg x d)] was given for 3 weeks in the experimental group and normal feeding in the control group. General condition of the chickens was observed after operation. The transplanted tendons were harvested from 4 chickens in each group for gross observation at 1, 2, 3, and 4 weeks after operation; the histological observation was performed at 1 and 3 weeks, and transmission electron microscope observation at 2 and 4 weeks. The blood and tendon were harvested from another 8 chickens in each group for flow cytometry and biomechanical tests respectively at 3 and 6 weeks.
All chickens survived to the experiment end. Gross observation: with time extending, hyperemia and edema around transplanted tendon were relieved. Rarefaction adhering zone was seen in experimental group, and pyknotic adhering zone in control group. Histological observation: inflammatory reaction in experimental group was slighter than that in control group at 1 and 3 weeks. Transmission electron microscope observation: at 2 and 4 weeks, fibroblasts had big cell nucleus, more euchromatin, and little heterochromatin in experimental group; however, there were small amount of rough endocytoplasmic reticulums with gentle expanded capsular space in control group, which contained sparse content. Flow cytometry test: at 3 and 6 weeks, peripheral blood contained less CD4+ and CD8+ T lymphocytes in experimental group than in control group, and the ratio of CD4+ to CD8+ T lymphocyte significantly decreased in experimental group when compared with control group (P < 0.05). Biomechanical examination: at 3 and 6 weeks, the maximum tensile strength in experimental group was bigger than that in control group, and tensile adhesion power in experimental group was smaller than that in control group. There were significant differences in the indexes between 2 groups (P < 0.05).
Triptolide can suppress immunological rejection reaction, strengthen tendon healing strength, and reduce tendon adhesion in allogenic tendon transplantation.
雷公藤甲素可抑制免疫排斥反应。探讨雷公藤甲素对鸡同种异体肌腱移植修复肌腱缺损的影响。
选取64只健康清洁级雄性来航鸡（4月龄，体重1.9 - 2.3 kg），建立第三趾肌腱缺损模型，行同种异体肌腱移植修复，随机分为2组（n = 32）。实验组给予雷公藤甲素喂养[100 μg/(kg·d)]，共3周，对照组正常喂养。术后观察鸡的一般情况。每组分别于术后1、2、3、4周取4只鸡的移植肌腱进行大体观察；于术后1周和3周进行组织学观察，术后2周和4周进行透射电镜观察。每组另取8只鸡，分别于术后3周和6周取血及肌腱进行流式细胞术检测和生物力学测试。
所有鸡均存活至实验结束。大体观察：随着时间延长，移植肌腱周围充血、水肿减轻。实验组可见黏附区稀疏，对照组可见黏附区固缩。组织学观察：术后1周和3周，实验组炎症反应较对照组轻。透射电镜观察：术后2周和4周，实验组成纤维细胞核大，常染色质增多，异染色质减少；对照组粗面内质网少量，囊腔轻度扩张，内容物稀疏。流式细胞术检测：术后3周和6周，实验组外周血CD4⁺和CD8⁺T淋巴细胞较对照组少，且实验组CD4⁺与CD8⁺T淋巴细胞比值较对照组显著降低（P < 0.05）。生物力学检测：术后3周和6周，实验组最大拉伸强度大于对照组，拉伸黏附力小于对照组。两组间各指标差异有统计学意义（P < 0.05）。
雷公藤甲素可抑制免疫排斥反应，增强肌腱愈合强度，减少同种异体肌腱移植中的肌腱粘连。
