Huba R, Hofmann H D
Max-Planck-Institut für Hirnforschung, Neuroanatomie, Frankfurt, F.R.G.
Neurosci Lett. 1990 Sep 4;117(1-2):37-42. doi: 10.1016/0304-3940(90)90116-q.
Purified neuronal monolayer cultures were prepared from embryonic chick retinae. Using immunocytochemistry for GABA and autoradiographic demonstration of [3H]muscimol uptake as markers, approximately 32% of the cells were identified as putative in vitro counterparts of gamma-aminobutyric acidergic (GABAergic) amacrine cells. Whole cell patch clamp recordings revealed close similarities between identified [3H]GABA accumulating neurons in culture and amacrine cells recorded in retinal slices, with respect to voltage-dependent membrane currents and to ion channels gated by exitatory (N-methyl-D-aspartate, kainate, quisqualate, glutamate) and inhibitory (GABA, glycine) amino acid agonists. The results demonstrate that retinal neurons are able to differentiate in dissociated retinal cultures. Such cultures may thus serve as a model system to study development and function of CNS neurons.
从鸡胚胎视网膜制备纯化的神经元单层培养物。使用针对γ-氨基丁酸（GABA）的免疫细胞化学和[3H]蝇蕈醇摄取的放射自显影作为标记，大约32%的细胞被鉴定为γ-氨基丁酸能（GABA能）无长突细胞在体外的对应物。全细胞膜片钳记录显示，在培养物中鉴定出的[3H]GABA积累神经元与视网膜切片中记录的无长突细胞在电压依赖性膜电流以及由兴奋性（N-甲基-D-天冬氨酸、海人藻酸、quisqualate、谷氨酸）和抑制性（GABA、甘氨酸）氨基酸激动剂门控的离子通道方面有密切相似性。结果表明，视网膜神经元能够在解离的视网膜培养物中分化。因此，这种培养物可作为研究中枢神经系统神经元发育和功能的模型系统。
