Zhang H, Wang J-W, Xu Y, Zhang K, Yi B, Sun J, Liu Y, Zhang X-M, Liu J-S
Department of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
J Int Med Res. 2012;40(3):943-53. doi: 10.1177/147323001204000313.
To investigate the effects of β-(3,4-dihydroxyphenyl)lactic acid on oxidative stress stimulated by high glucose levels in human peritoneal mesothelial cells (HPMCs) in vitro.
HPMCs were incubated with 100 mol/l glucose followed by 0.625-20 mg/ml β-(3,4-dihydroxyphenyl)lactic acid. Reactive oxygen species (ROS) were quantified by flow cytometry. Relative levels of fibronectin-1 (FN1), collagen-I α(1) (COL1A1), endothelin-1 (EDN1) and haem oxygenase-1 (HMOX1) mRNA and protein were quantified by real-time reverse transcription-polymerase chain reaction and Western blotting, respectively. Absolute levels of FN1 and COLIA1 were quantified by enzyme-linked immunosorbent assay.
β-(3,4-Dihydroxyphenyl)lactic acid significantly decreased ROS levels, and EDN1 mRNA and protein levels, in dose- and time-dependent manners. HMOX1 mRNA and protein levels were significantly increased by β-(3,4-dihydroxyphenyl)lactic acid in dose-dependent manners. COL1A1 and FN1 mRNA and protein levels were significantly decreased by β-(3,4-dihydroxyphenyl)lactic acid in dose- and time-dependent manners.
β-(3,4-Dihydroxyphenyl)lactic acid inhibited oxidative stress and reversed increases in FN1 and COLIA1 induced by high glucose levels in HPMCs. This may contribute to a protective role in peritoneal fibrosis.
研究β-（3,4-二羟基苯基）乳酸对体外培养的人腹膜间皮细胞（HPMCs）中高糖诱导的氧化应激的影响。
将HPMCs与100 mol/l葡萄糖孵育，随后加入0.625 - 20 mg/mlβ-（3,4-二羟基苯基）乳酸。通过流式细胞术对活性氧（ROS）进行定量分析。分别通过实时逆转录-聚合酶链反应和蛋白质免疫印迹法对纤连蛋白-1（FN1）、I型胶原α(1)（COL1A1）、内皮素-1（EDN1）和血红素加氧酶-1（HMOX1）的mRNA和蛋白质的相对水平进行定量分析。通过酶联免疫吸附测定法对FN1和COLIA1的绝对水平进行定量分析。
β-（3,4-二羟基苯基）乳酸以剂量和时间依赖性方式显著降低ROS水平、EDN1 mRNA和蛋白质水平。β-（3,4-二羟基苯基）乳酸以剂量依赖性方式显著增加HMOX1 mRNA和蛋白质水平。β-（3,4-二羟基苯基）乳酸以剂量和时间依赖性方式显著降低COL1A1和FN1 mRNA及蛋白质水平。
β-（3,4-二羟基苯基）乳酸抑制氧化应激，并逆转HPMCs中高糖诱导的FN1和COLIA1的增加。这可能对腹膜纤维化起到保护作用。
