Department of Pharmacology and Physiology, Pasteur Institute of Iran, Tehran, Iran.
Pharm Biol. 2012 Nov;50(11):1416-22. doi: 10.3109/13880209.2012.682118. Epub 2012 Sep 11.
Currently, natural products have been shown to present interesting biological and pharmacological activities and are used as chemotherapeutic agents. Plants have historically been used in treating cancer and are recognized for their ability to produce secondary metabolites. Juglans regia L. (Juglandaceae) has medicinal applications to treat a wide range of diseases such as cancer.
The current study was designed to evaluate the antiproliferative activity of total extract as well as several fractions from the leaves of J. regia.The total phenolics, flavonoids, and condensed tannins content of these extracts were also determined to obtain further information on the correlation between the contents of phenolic compounds and antiproliferative effects as well as the leaf developmental stages.
Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry methods against human oral cancer, breast adenocarcinoma and colon adenocarcinoma cell lines. The total phenolics, flavonoids, and condensed tannins were determined by Folin-Ciocalteu, aluminum chloride and butanol-HCl colorimetric methods.
Our present study has shown that chloroform fraction has the lowest IC(50) values (0.36-0.81 mg/mL) and also induces cell cycle arrest (G0\G1 phase) after a 24 h treatment. The colorimetric methods showed the highest amount of total phenolics, flavonoids, and condensed tannins in the methanol fraction (120.28 ± 2.32, 59.44 ± 0.87, 227.00 ± 4.91 mg/g of dry weight of extract).
The results obtained herein indicate that walnut chloroform fraction may contain effective compounds which can be used as a chemotherapeutic agent.
目前，天然产物已被证明具有有趣的生物和药理活性，并被用作化疗药物。植物在治疗癌症方面有着悠久的历史，并且因其能够产生次生代谢物而受到认可。胡桃（Juglans regia L.，胡桃科）具有药用价值，可用于治疗多种疾病，如癌症。
本研究旨在评估胡桃叶总提取物及几种馏分的抗增殖活性。还测定了这些提取物中总酚类、类黄酮和缩合单宁的含量，以获得关于酚类化合物含量与抗增殖作用以及叶片发育阶段之间相关性的进一步信息。
采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐和流式细胞术方法，评价人口腔癌、乳腺癌和结肠癌细胞系的抗增殖活性。采用 Folin-Ciocalteu、三氯化铝和丁醇-HCl 比色法测定总酚类、类黄酮和缩合单宁的含量。
本研究表明，氯仿馏分具有最低的 IC50 值（0.36-0.81 mg/mL），并且在 24 小时处理后还能诱导细胞周期停滞（G0/G1 期）。比色法显示甲醇馏分中总酚类、类黄酮和缩合单宁的含量最高（120.28±2.32、59.44±0.87、227.00±4.91 mg/g 提取物干重）。
本研究结果表明，胡桃氯仿馏分可能含有可作为化疗药物的有效化合物。
