US Environmental Protection Agency, National Exposure Research Laboratory, Las Vegas, NV 89193-3478, USA.
Chemosphere. 2013 Jan;90(1):1-6. doi: 10.1016/j.chemosphere.2012.06.053. Epub 2012 Aug 18.
An immunoaffinity chromatography (IAC) column was developed as a simple cleanup procedure for preparing environmental samples for analysis of polychlorinated biphenyls (PCBs). Soil and sediment samples were prepared using pressurized liquid extraction (PLE), followed by the IAC cleanup, with detection by an enzyme-linked immunosorbent assay (ELISA). Quantitative recoveries (84-130%) of PCB-126 were obtained in fortified sediment and soil samples using the PLE/IAC/ELISA method. These results demonstrated that the IAC procedure effectively removed interferences from the soil and sediment matrices. The IAC column could be reused more than 20 times with no change in performance with 99.9% methanol/0.1% Triton X-100 as the elution solvent. Results of 17 soil and sediment samples prepared by PLE/IAC/ELISA correlated well with those obtained from a conventional multi-step cleanup with gas chromatography/mass spectrometry detection.
建立了一种免疫亲和色谱（IAC）柱，作为一种简单的净化程序，用于准备环境样品以分析多氯联苯（PCBs）。使用加压液体萃取（PLE）制备土壤和沉积物样品，然后进行 IAC 净化，并用酶联免疫吸附测定（ELISA）进行检测。使用 PLE/IAC/ELISA 方法，在添加 PCB-126 的沉积物和土壤样品中获得了 84-130%的定量回收率。这些结果表明，IAC 程序有效地去除了土壤和沉积物基质中的干扰物。用 99.9%甲醇/0.1%Triton X-100 作为洗脱溶剂，IAC 柱可以重复使用 20 多次以上，性能没有变化。通过 PLE/IAC/ELISA 制备的 17 个土壤和沉积物样品的结果与通过传统的多步净化与气相色谱/质谱检测获得的结果高度相关。
