Guinec N, Dalet-Fumeron V, Pagano M
Laboratoire de Biochimie, UFR Médicale Broussais-Hôtel Dieu, Université Pierre et Marie-Curie, Paris, France.
Pathol Biol (Paris). 1990 Dec;38(10):988-92.
Lysosomal cysteine proteinases, cathepsins B, H and L, and tumor cathepsin-B-like protease, are capable of digesting bovine lens capsule, a typical basement membrane, in vitro. Cathepsins L and H proved most active in the pH range 5.0-7.0, whereas cathepsin B and cathepsin B-like protease showed greatest activity at pH 3.0. The process was slow and involved binding of the proteases to the basement membrane. These proteases, of which some are produced by malignant cells, may contribute to tumor spread and progression to metastatic disease.
溶酶体半胱氨酸蛋白酶、组织蛋白酶B、H和L以及肿瘤组织蛋白酶B样蛋白酶，在体外能够消化典型的基底膜——牛晶状体囊膜。组织蛋白酶L和H在pH值5.0 - 7.0范围内活性最强，而组织蛋白酶B和组织蛋白酶B样蛋白酶在pH 3.0时活性最高。该过程较为缓慢，且涉及蛋白酶与基底膜的结合。这些蛋白酶中有些由恶性细胞产生，可能有助于肿瘤扩散以及进展为转移性疾病。
