Scholler Nathalie
Center for Research on Reproduction and Women's Health, Penn Ovarian Cancer Research Center, University of Pennsylvania School of Medicine, Philadelphia, PA, USA,
Methods Mol Biol. 2012;907:259-80. doi: 10.1007/978-1-61779-974-7_15.
The critical need for renewable, high-quality affinity reagents in biological research, as well as for diagnostic and therapeutic applications, has required the development of new platforms of discovery. Yeast display is one of the main methods of in vitro display technology with phage display. Yeast display has been chosen by numerous groups to refine both affinity and specificity of antibodies because it enables fine discrimination between mutant clones of similar affinity. In addition, the construction of display libraries of antibody fragments in yeast permit to sample the immune antibody repertoire more fully than using phage. This chapter gives an updated overview of the available systems of yeast display platforms and libraries, followed up by technical descriptions of selection methods of antibody fragments by yeast display.
在生物学研究以及诊断和治疗应用中，对可再生、高质量亲和试剂的迫切需求推动了新发现平台的发展。酵母展示是体外展示技术的主要方法之一，与噬菌体展示类似。许多研究团队选择酵母展示来优化抗体的亲和力和特异性，因为它能够对亲和力相似的突变克隆进行精细区分。此外，在酵母中构建抗体片段展示文库比使用噬菌体更能充分地筛选免疫抗体库。本章将对现有的酵母展示平台和文库系统进行更新概述，随后对通过酵母展示筛选抗体片段的方法进行技术描述。
