Borgel Julie, Guibert Sylvain, Weber Michael
Institute of Molecular Genetics, UMR 5535, Université Montpellier 2, Université Montpellier 1, CNRS, Montpellier, France.
Methods Mol Biol. 2012;925:149-58. doi: 10.1007/978-1-62703-011-3_9.
Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing approach for unbiased enrichment of DNA that is methylated on cytosines. The principle is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody that specifically recognizes 5-methylcytidine (5mC), which can be combined with PCR or high-throughput analysis (microarrays, deep sequencing). The MeDIP technique has been originally used to generate DNA methylation profiles on a genome scale in mammals and plants. Here we provide an optimized version of the MeDIP protocol suitable for low amounts of DNA, which can be used to study DNA methylation in cellular populations available in small quantities.
甲基化DNA免疫沉淀（MeDIP）是一种用于无偏差富集胞嘧啶甲基化DNA的免疫捕获方法。其原理是通过超声随机剪切基因组DNA，并用特异性识别5-甲基胞嘧啶（5mC）的抗体进行免疫沉淀，该方法可与PCR或高通量分析（微阵列、深度测序）相结合。MeDIP技术最初用于在哺乳动物和植物中生成全基因组范围内的DNA甲基化图谱。在此，我们提供了一种适用于少量DNA的MeDIP方案优化版本，可用于研究少量细胞群体中的DNA甲基化。
