经自动免疫分析法检测马眼泪中的妥布霉素的药代动力学分析。

Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay.

机构信息

Department of Veterinary Clinical and Diagnostic Sciences, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada.

出版信息

BMC Vet Res. 2012 Aug 21;8:141. doi: 10.1186/1746-6148-8-141.

DOI:10.1186/1746-6148-8-141

PMID:22909398

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3489562/

Abstract

BACKGROUND

Ophthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoassay analysis.

RESULTS

The mean tobramycin concentrations in the tears at 5, 10, 15, 30 minutes and 1, 2, 4, 6 hours after administration were 759 (±414), 489 (±237), 346 (±227), 147 (±264), 27.6 (±28.4), 14.8 (±66.6), 6.7 (±18.6), and 23.4 (±73.4) mg/L. Mean tobramycin concentration was maintained above the MIC90 for commonly isolated bacteria for 68.5 min.

CONCLUSION

A single dose of topical tobramycin resulted in therapeutic concentrations of tobramycin in the tears for 1 h after administration. Therapeutic levels of tobramycin remained in equine tears 6 times longer than was reported in rabbit tears.

摘要

背景

由于缺乏药代动力学研究，大型动物的眼科抗生素治疗通常是经验性的。本研究的目的是使用自动免疫分析来确定正常马中 0.3%妥布霉素滴眼液在泪液中的药代动力学。

结果

滴药后 5、10、15、30 分钟和 1、2、4、6 小时，妥布霉素在泪液中的平均浓度分别为 759（±414）、489（±237）、346（±227）、147（±264）、27.6（±28.4）、14.8（±66.6）、6.7（±18.6）和 23.4（±73.4）mg/L。妥布霉素的平均浓度维持在 MIC90 以上，这是常见分离细菌的浓度，持续 68.5 分钟。