Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, via Celoria 10, 20133 Milano, Italy.
Vet J. 2013 Apr;196(1):47-51. doi: 10.1016/j.tvjl.2012.07.022. Epub 2012 Aug 19.
α1-Acid glycoprotein (AGP) is an acute phase protein that modulates innate immunity and increases in response to infection or injury. The effects of native (phosphorylated) and partially dephosphorylated AGP on the antimicrobial activities of bovine polymorphonuclear leucocytes (PMNs) and monocytes were evaluated. Native AGP inhibited phagocytosis and killing of Escherichia coli by PMNs and monocytes. Engulfment and killing of E. coli were reduced at the acute phase concentration of AGP (0.9 mg/mL) compared with a physiological concentration (0.3mg/mL). The ability of AGP to inhibit phagocytosis by monocytes and the killing of E. coli by PMNs was reduced following dephosphorylation. The findings indicate that the functions of PMNs and monocytes are differentially regulated by varying concentrations of AGP and its phosphorylation state.
α1-酸性糖蛋白（AGP）是一种急性期蛋白，可调节先天免疫，并在感染或损伤时增加。评估天然（磷酸化）和部分去磷酸化的 AGP 对牛多形核白细胞（PMN）和单核细胞的抗菌活性的影响。天然 AGP 抑制 PMN 和单核细胞吞噬和杀死大肠杆菌。与生理浓度（0.3mg/mL）相比，AGP（0.9mg/mL）的急性期浓度可降低对大肠杆菌的吞噬和杀伤作用。AGP 抑制单核细胞吞噬作用和 PMN 杀死大肠杆菌的能力在去磷酸化后降低。研究结果表明，PMN 和单核细胞的功能受 AGP 浓度及其磷酸化状态的不同调节。
