[两种DNA提取方法的比较分析]
[Comparative analysis of 2 methods of DNA extraction].
作者信息
Sánchez García I, Martín Seisdedos C, Pérez Grande R, Corral de la Calle J, Rodríguez Arias C, González-Sarmiento R
机构信息
Departamento de Medicina, Hospital Clínico Universitario, Salamanca.
出版信息
Sangre (Barc). 1990 Oct;35(5):405-7.
The study of the organization of immunoglobulin and T cell receptor genes in leukaemias and lymphomas depends on our ability to obtain high molecular weight DNA. We have compared two different methods of DNA extraction: one being enzymatic, using the enzyme proteinase K, and the other chemical, using urea as a substance purifying DNA. Since the amount and purity of the DNA obtained are similar with each of the two methods of DNA extraction, both are valid to make such a genetic analysis.
白血病和淋巴瘤中免疫球蛋白及T细胞受体基因组织的研究取决于我们获取高分子量DNA的能力。我们比较了两种不同的DNA提取方法：一种是酶法，使用蛋白酶K；另一种是化学法，使用尿素作为纯化DNA的物质。由于两种DNA提取方法所获得的DNA量和纯度相似，因此两种方法都适用于进行此类基因分析。
Zotero 插件