Institute of Science, Department of Biotechnology, Anadolu University, Eskişehir, Turkey.
Phytother Res. 2013 Jun;27(6):900-5. doi: 10.1002/ptr.4804. Epub 2012 Aug 21.
Extracts of Aesculus hippocastanum L. (horse chestnut) seed have been used in the treatment of chronic venous insufficiency, edema and hemorrhoids. Most of the beneficial effects of horse chestnut are attributed to its principal component β-escin or escin. We have evaluated the cytotoxic and apoptotic effects of escin in the H-Ras 5RP7 cell line by analyzing cell growth inhibition, apoptosis and caspase-3 dependent activity. We have also shown structural and ultrastructural changes in these cell using confocal and transmission electron microscopy. The results indicated that escin has significant inhibitory effects on cell growth and the percentage of apoptotic cells increased after treatment with escin, and the micrographs confirmed that escin damaged these cells and induced apoptosis.
欧洲七叶树（马栗树）的种子提取物被用于治疗慢性静脉功能不全、水肿和痔疮。马栗树的大部分有益作用归因于其主要成分 β-七叶皂苷或七叶皂苷。我们通过分析细胞生长抑制、细胞凋亡和 caspase-3 依赖性活性，评估了七叶皂苷在 H-Ras 5RP7 细胞系中的细胞毒性和促凋亡作用。我们还使用共聚焦和透射电子显微镜显示了这些细胞的结构和超微结构变化。结果表明，七叶皂苷对细胞生长有显著的抑制作用，用七叶皂苷处理后，凋亡细胞的比例增加，显微镜照片证实七叶皂苷破坏了这些细胞并诱导了细胞凋亡。
