Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou, Zhejiang 310021, China.
Poult Sci. 2012 Sep;91(9):2270-4. doi: 10.3382/ps.2012-02149.
Abundance of duck adipocyte fatty acid-binding protein (A-FABP) mRNA was detected in this study by quantitative real-time PCR. The results showed that duck A-FABP gene was expressed in muscular tissues and many organs, except pancreas, lung, and kidney, and highly expressed in adipose tissues, especially in sebum. The expression of A-FABP and adipocyte differentiation-related genes was upregulated by oleic acid, and the A-FABP knockdown suppressed the oleic acid-stimulated expression of these genes in the cultured duck adipocytes, indicating A-FABP might play an important role in duck adipocyte differentiation.
本研究通过定量实时 PCR 检测到鸭脂肪细胞脂肪酸结合蛋白 (A-FABP) mRNA 的丰度。结果表明，鸭 A-FABP 基因在肌肉组织和许多器官（胰腺、肺和肾除外）中表达，在脂肪组织中高度表达，特别是皮脂中。油酸上调 A-FABP 和脂肪细胞分化相关基因的表达，而 A-FABP 的敲低抑制了培养的鸭脂肪细胞中这些基因受油酸刺激的表达，表明 A-FABP 可能在鸭脂肪细胞分化中发挥重要作用。
