College of Animal Science and Technology, Northeast Agricultural University, Harbin, PR China.
Comp Biochem Physiol B Biochem Mol Biol. 2010 Dec;157(4):357-63. doi: 10.1016/j.cbpb.2010.08.005. Epub 2010 Aug 20.
Fatty acid-binding proteins (FABPs) may facilitate the diffusion of fatty acids within the cytoplasm. Adipocyte FABP (A-FABP) null mice exhibit altered rates of lipolysis, and reprogrammed expression of genes related to lipid metabolism. In the current study, chicken adipocytes with short hairpin RNA-mediated knockdown and cDNA overexpression of A-FABP were used to investigate the role of chicken A-FABP in lipid metabolism of adipocytes. The results showed that there was no significant change of lipid metabolism in the A-FABP interference adipocytes. However, in the A-FABP overexpression adipocytes, the lipolysis was increased significantly at 36 h (p<0.05), and lipid accumulation was increased significantly at 48, 60 and 72 h (p<0.05). The expression of PPAR γ and perilipin genes were upregulated, while the expression of E-FABP gene was downregulated after transfection with pcDNA3.1/A-FABP (p<0.05). The present study suggested that A-FABP gene might affect the lipid metabolism through the PPAR γ pathway, and the function between A-FABP and E-FABP gene was compensatory in the chickens.
脂肪酸结合蛋白（FABPs）可能有助于脂肪酸在细胞质内扩散。脂肪细胞 FABP（A-FABP）缺失小鼠表现出脂解率的改变，以及与脂质代谢相关基因的重新编程表达。在本研究中，使用短发夹 RNA 介导的敲低和 cDNA 过表达 A-FABP 的鸡脂肪细胞来研究鸡 A-FABP 在脂肪细胞脂质代谢中的作用。结果表明，A-FABP 干扰脂肪细胞的脂质代谢没有明显变化。然而，在 A-FABP 过表达脂肪细胞中，36 h 时脂解明显增加（p<0.05），48、60 和 72 h 时脂质积累明显增加（p<0.05）。转染 pcDNA3.1/A-FABP 后，PPAR γ 和 perilipin 基因的表达上调，而 E-FABP 基因的表达下调（p<0.05）。本研究表明，A-FABP 基因可能通过 PPAR γ 途径影响脂质代谢，并且 A-FABP 和 E-FABP 基因之间的功能在鸡中具有代偿性。
