Institute for Research in Molecular Medicine (INFORMM), University Sains Malaysia, USM, Penang, Malaysia.
Eur Rev Med Pharmacol Sci. 2012 Aug;16(8):1028-32.
To determine the usefulness of prednisolone in increasing the number of Toxoplasma (T.) gondii tachyzoites and bradyzoites in mice.
The mice were water-fasted prior to being immunosuppressed with oral inoculation of prednisolone. Tachyzoites of 7T gondii RH strain were inoculated into mice and the number of the parasites in the intraperitoneal fluids was then determined at 96 hs post-infection. In addition, tachyzoites of T. gondii ME49 strains were orally introduced into mice and the number of brain cysts formed was observed by microscopic observation at 45 days post-infection.
T. gondii propagation was found to be significantly improved by introduction of the prednisolone (p = 0.0004); and the number of parasite showed positive correlation with the increment in dosage of prednisolone (r = 0.9051).
The use of prednisolone greatly improved the number of parasite formed in mice: both tachyzoite and cyst forms.
确定泼尼松龙在增加小鼠弓形虫速殖子和缓殖子数量方面的作用。
在对小鼠进行免疫抑制的过程中，采用口服泼尼松龙进行水饥饿处理。将 7T 弓形虫 RH 株的速殖子接种到小鼠体内，然后在感染后 96 小时测定腹腔液中的寄生虫数量。此外，将 T. gondii ME49 株的速殖子经口引入小鼠体内，并在感染后 45 天通过显微镜观察观察脑囊形成的数量。
泼尼松龙的引入显著促进了弓形虫的繁殖（p = 0.0004）；寄生虫数量与泼尼松龙剂量的增加呈正相关（r = 0.9051）。
泼尼松龙的使用大大增加了小鼠体内形成的寄生虫数量：包括速殖子和缓殖子。
