Lifeline Multispecialty Hospital, 5/639, Rajiv Gandhi Salai, OMR, Perungudi, Chennai, India.
Cell Biol Int. 2012 Nov 1;36(11):1029-36. doi: 10.1042/CBI20120201.
Recent scientific explorations in search of novel sources for autologous transplantation transpired an alternative source of MSCs (mesenchymal stem cells) derived from omentum fat. The scarcity of experimental evidences probing into the biosafety concerns of omentum fat-derived MSC under prolonged culture conditions limits its applicability as an efficient tool in regenerative medicine. This study, thus, aims to optimize human omentum fat-derived MSC in four different media [DMEM (Dulbecco's modified Eagle's medium) LG (low glucose), DMEM KO (knock out), α-MEM (α-minimal essential media) and DMEM F12] in the facets of phenotypic characterization, growth kinetics, differentiation and karyotyping under prolonged culture. The cells exhibited a similarity in expression profile for the majority of markers with evidential variations in certain markers. The relevance of omentum fat-derived MSCs became evident from its triumphant differentiation potential and karyotypic stability substantiated even at later passage. The results obtained from growth curve and PDT (population doubling time) lead to optimization of appropriate media for omentum fat-derived stem cell research, thereby bringing omentum fat into the forefront of regenerative medicine.
最近在寻找自体移植新来源的科学探索中，出现了一种源自网膜脂肪的间充质干细胞（MSC）的替代来源。由于缺乏实验证据来探究在长时间培养条件下网膜脂肪来源的 MSC 的生物安全性问题，限制了其作为再生医学中有效工具的适用性。因此，本研究旨在通过优化四种不同培养基（DMEM（Dulbecco 修改的 Eagle 培养基）LG（低糖）、DMEM KO（敲除）、α-MEM（α-最小必需培养基）和 DMEM F12）中的人网膜脂肪来源 MSC，从表型特征、生长动力学、分化和染色体核型等方面进行研究。细胞在大多数标志物的表达谱上表现出相似性，但在某些标志物上存在明显的差异。从其成功的分化潜力和染色体核型稳定性来看，网膜脂肪来源的 MSC 具有重要意义，即使在后期传代中也得到了证实。从生长曲线和 PDT（群体倍增时间）获得的结果为网膜脂肪来源干细胞的研究优化了合适的培养基，从而使网膜脂肪成为再生医学的前沿领域。
