The Institute for Advanced Materials and Nano Biomedicine, Tongji University, 67 Chifeng Road, Shanghai, 200092, China.
Biomacromolecules. 2012 Sep 10;13(9):2723-30. doi: 10.1021/bm3006903. Epub 2012 Aug 31.
RNA interference is one of the most promising technologies for cancer therapeutics, while the development of a safe and effective small interfering RNA (siRNA) delivery system is still challenging. Here, amphipol polymer and protamine peptide were employed to modify magnetic nanoparticles to form cell-penetrating magnetic nanoparticles (CPMNs). The unique CPMN could efficiently deliver the eGFP siRNA intracellularly and silence the eGFP expression in cancer cells, which was verified by fluorescent imaging of cancer cells. Compared with lipofectamine and polyethyleneimine (PEI), CPMNs showed superior silencing efficiency and biocompatibility with minimum siRNA concentration as 5 nm in serum-containing medium. CPMN was proved to be an efficient siRNA delivery system, which will have great potential in applications as a universal transmembrane carrier for intracellular gene delivery and simultaneous MRI imaging.
RNA 干扰是癌症治疗中最有前途的技术之一，而开发安全有效的小干扰 RNA（siRNA）传递系统仍然具有挑战性。在这里，两性聚合物和鱼精蛋白肽被用来修饰磁性纳米颗粒，形成穿透细胞的磁性纳米颗粒（CPMNs）。独特的 CPMN 可以有效地将 eGFP siRNA 递送到癌细胞内，并使癌细胞中的 eGFP 表达沉默，这通过癌细胞的荧光成像得到了验证。与脂质体和聚乙烯亚胺（PEI）相比，CPMNs 在含血清的培养基中以 5nm 的最小 siRNA 浓度显示出更高的沉默效率和生物相容性。CPMN 被证明是一种有效的 siRNA 传递系统，它将作为一种通用的跨膜载体，在细胞内基因传递和同时进行 MRI 成像方面具有巨大的应用潜力。
