Drews U, Mengis W
Anatomisches Institut, Tübingen, Federal Republic of Germany.
Anat Embryol (Berl). 1990;182(5):447-54. doi: 10.1007/BF00178909.
The mechanism of excitation contraction coupling during morphogenetic movements is unknown. We describe a contraction wave in the chick blastoderm after muscarinic stimulation, which indicates that an autocrine cholinergic mechanism might be involved in the induction of morphogenetic movements during embryogenesis. Chick blastoderms were explanted in a modified new culture and the cellular movements were recorded by time lapse video filming. Perfusion with acetylcholine or carbachol induced a contraction wave in the blastoderm which started in the periphery at the point of entrance of the drug, and proceeded within 8-10 min through the area pellucida to the opposite side of the blastoderm. Perfusion with the muscarinic antagonist pilocarpine in turn induced relaxation. Atropine inhibited the effect of the agonists acetylcholine and carbachol. From earlier studies we know that in the chick embryo a muscarinic system is present, the expression of which correlates with morphogenetic movements. The induction of a contraction wave in the chick blastoderm by muscarinic agonists supports our hypothesis that embryonic cell movements might be regulated via muscarinic receptors.
形态发生运动过程中的兴奋收缩偶联机制尚不清楚。我们描述了毒蕈碱刺激后鸡胚盘的收缩波，这表明自分泌胆碱能机制可能参与胚胎发育过程中形态发生运动的诱导。将鸡胚盘接种于改良的新培养基中，通过延时视频拍摄记录细胞运动。用乙酰胆碱或卡巴胆碱灌注可诱导胚盘出现收缩波，该收缩波始于药物进入部位的周边，在8 - 10分钟内穿过透明区到达胚盘的另一侧。用毒蕈碱拮抗剂毛果芸香碱灌注则诱导松弛。阿托品抑制激动剂乙酰胆碱和卡巴胆碱的作用。从早期研究中我们知道，在鸡胚中存在一个毒蕈碱系统，其表达与形态发生运动相关。毒蕈碱激动剂在鸡胚盘中诱导收缩波支持了我们的假设，即胚胎细胞运动可能通过毒蕈碱受体进行调节。
