Penner Philip L, Brick Irving
Department of Science, Borough of Manhattan Community College, 199 Chambers St., 10007, N.Y., N.Y., USA.
Department of Biology, New York University, 951 Brown Building, Washington Sq., 10003, N.Y., N.Y., USA.
Wilehm Roux Arch Dev Biol. 1984 Jul;193(4):234-241. doi: 10.1007/BF01260344.
Cholinesterase histochemistry and SEM were performed on whole chick blastoderms, stage 4 Hamburger-Hamilton, to study the relationship between acetylcholinesterase (AChE) and cell movement in the epiblast. Correlation of LM photomicrographs of enzyme sites with SEM micrographs of surface topography permitted the determination of the three dimensional morphology of enzyme-positive cells. On the epiblast under surface two cell configurations were observed indicating movement of cells out of the epiblast at sites distant to the streak. In one configuration, cells are flask-shaped with tapered tails. In the other configuration, roughly spherical cells seem to be loosening contact with mounds that bulge from the epiblast. Flask cells and detaching mound cells are AChE-positive. On the upper surface of the hypoblast some cells are spherical and attached to the surface by thin cytoplasmic processes while others are flattened and elongated. The former are intensely stained for AChE and the latter are faintly stained or unstained. Ultrastructural studies indicate the presence of AChE at discontinuous sites in the perikaryon, endoplasmic reticulum and golgi. Reaction product was observed at the aforementioned sites when acetylthiocholine iodide was the substrate but not when butyrylthiocholine iodide was used or substrate was omitted. The results support the use of AChE as an endogenous marker of cell movement and provide morphological information on the process of polyingression.
对处于第4期汉堡-汉密尔顿（Hamburger-Hamilton）阶段的整个鸡胚盘进行胆碱酯酶组织化学和扫描电子显微镜（SEM）研究，以探讨上胚层中乙酰胆碱酯酶（AChE）与细胞运动之间的关系。通过酶位点的光学显微镜照片与表面形貌的扫描电子显微镜照片的相关性，得以确定酶阳性细胞的三维形态。在上胚层下表面观察到两种细胞形态，表明细胞在远离原条的部位从上胚层移出。一种形态中，细胞呈烧瓶状，尾部逐渐变细。另一种形态中，大致呈球形的细胞似乎正在与从上胚层隆起的丘状结构松开接触。烧瓶状细胞和脱离丘状结构的细胞为AChE阳性。在下胚层上表面，一些细胞呈球形，通过细的细胞质突起附着于表面，而另一些细胞则扁平且细长。前者AChE染色强烈，后者染色微弱或未染色。超微结构研究表明，在核周、内质网和高尔基体的不连续部位存在AChE。当以碘化乙酰硫代胆碱为底物时，在上述部位观察到反应产物，但使用碘化丁酰硫代胆碱为底物或省略底物时则未观察到。这些结果支持将AChE用作细胞运动的内源性标记，并提供了关于多内陷过程的形态学信息。
