Romero Belen Tejada, Evans Deborah J, Aboobaker A Aziz
Centre for Genetics and Genomics, Queen's Medical Centre, University of Nottingham, Nottingham, UK.
Methods Mol Biol. 2012;916:167-79. doi: 10.1007/978-1-61779-980-8_13.
Planarians provide a relatively simple model system in which to study stem cell dynamics and regenerative phenomena. As with other systems understanding the dynamics of stem cell and stem cell progeny is crucial in order to get at the molecular mechanisms orchestrating stem cell biology. Planarians have an abundant adult stem cell population that can be observed using Fluorescence-Activated Cell Sorting (FACS). This approach allows different subpopulations of stem cells and their progeny to be monitored and sorted for downstream studies in response to different regenerative scenarios, drug treatments, or RNAi knockdown of genes required for regenerative events.
涡虫提供了一个相对简单的模型系统，用于研究干细胞动力学和再生现象。与其他系统一样，了解干细胞及其子代的动力学对于揭示调控干细胞生物学的分子机制至关重要。涡虫拥有丰富的成体干细胞群体，可通过荧光激活细胞分选（FACS）进行观察。这种方法能够监测和分选不同亚群的干细胞及其子代，以便针对不同的再生情况、药物处理或再生事件所需基因的RNA干扰敲低进行下游研究。
