Mahdipour Elahe, Mace Kimberly A
Faculty of Life Sciences,The Healing Foundation Centre, University of Manchester, Manchester, UK.
Methods Mol Biol. 2012;916:219-29. doi: 10.1007/978-1-61779-980-8_17.
Analysis of tissue repair and regeneration in a variety of organisms has demonstrated that stem and progenitor cells play a critical role in the healing and regenerative response. In particular, during cutaneous wound healing bone marrow-derived cells are recruited to the site of injury in large numbers, often comprising over 50% of the cells within the wound milieu. These bone marrow-derived cells are comprised mostly of a heterogeneous mix of myeloid cells. In the early stages of wound healing, the most prominent subtypes are Gr-1(+)CD11b(+) cells that consist of progenitor cells and more differentiated granulocytes. Under certain conditions, these cells have the potential to strongly promote angiogenesis, and thus tissue repair and regeneration. This chapter provides methods by which one can isolate these cells from wound tissue and assess their pro-angiogenic capacity via gene expression analyses and functional in vivo angiogenesis assays.
对多种生物体中的组织修复和再生进行分析表明，干细胞和祖细胞在愈合和再生反应中起着关键作用。特别是在皮肤伤口愈合过程中，大量骨髓来源的细胞被募集到损伤部位，通常占伤口环境中细胞的50%以上。这些骨髓来源的细胞主要由髓样细胞的异质混合物组成。在伤口愈合的早期阶段，最突出的亚型是Gr-1(+)CD11b(+)细胞，它由祖细胞和分化程度更高的粒细胞组成。在某些条件下，这些细胞具有强烈促进血管生成的潜力，从而促进组织修复和再生。本章提供了从伤口组织中分离这些细胞并通过基因表达分析和体内血管生成功能测定来评估其促血管生成能力的方法。
