Department of Basic Veterinary Medical Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan.
Arch Virol. 2012 Dec;157(12):2343-8. doi: 10.1007/s00705-012-1447-z. Epub 2012 Aug 23.
Bovine leukemia virus (BLV) is distributed worldwide. BLV has many effects on the health status and productivity of infected animals and is a potential risk for humans. In this study, we aimed to investigate the presence of and genotype bovine leukemia viruses on Jordanian dairy farms. Nested PCR coupled with RFLP and direct sequencing of a partial fragment of the env gene were carried out. Two BLV genotypes were found, genotypes 1 and 6. These genotypes were identified by nested PCR-RFLP of 444 bp of the env gene by restriction digestion with HaeIII, Bcl I and Pvu II. However, BLV-Jordan-10 seems to represent an entirely new genotype in our phylogenetic analysis. The nucleotide sequence identity between these two Jordanian BLV genotypes (1 and 6) was 96.2 %. The nucleotide sequence identity between Jordanian BLV genotype 1 and other reference BLV genotype 1 strains ranged from 99 % to 99.5 %. The nucleotide sequence similarity of the Jordanian BLV genotype 6 to other BLV genotypes ranged from 90 % to 96.7 %. A neutralizing motif and CD8(+) T-cell epitope were found in the env protein of both Jordanian isolates. In this study, we documented the presence of two BLV genotypes (1 and 6) on Jordanian dairy farms.
牛白血病病毒（BLV）分布于全球。BLV 对感染动物的健康状况和生产力有许多影响，是人类的潜在风险。本研究旨在调查约旦奶牛场牛白血病病毒的存在和基因型。我们进行了巢式 PCR 结合 RFLP 和 env 基因部分片段的直接测序。发现了两种 BLV 基因型，基因型 1 和 6。这些基因型是通过 HaeIII、BclI 和 PvuII 限制性消化对 env 基因的 444bp 进行巢式 PCR-RFLP 鉴定的。然而，BLV-Jordan-10 在我们的系统发育分析中似乎代表了一种全新的基因型。这两种约旦 BLV 基因型（1 和 6）之间的核苷酸序列同一性为 96.2%。约旦 BLV 基因型 1 与其他参考 BLV 基因型 1 株之间的核苷酸序列同一性为 99%至 99.5%。约旦 BLV 基因型 6 与其他 BLV 基因型的核苷酸相似性为 90%至 96.7%。在两种约旦分离株的 env 蛋白中发现了一个中和基序和 CD8+T 细胞表位。本研究记录了约旦奶牛场存在两种 BLV 基因型（1 和 6）。
