State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, China.
J Basic Microbiol. 2013 Jun;53(6):549-54. doi: 10.1002/jobm.201100532. Epub 2012 Aug 23.
The effect of decomposition of Microcystis blooms on the sediment bacterial communities was investigated in a 14-day mesocosm experiments under dark and anoxic condition with three differing levels of Microcystis spp. biomass (from 20 to 2000 µg L(-1)) as measured by chlorophyll-a (Chl-a) concentration in the water column. Sediment samples were collected on days 0 and 14 for bacterial community composition (BCC) analysis, based on rapid decreases in the Chl-a concentration of water column. The BCC was determined by denaturing gradient gel electrophoresis and sequencing of predominant bands. The results showed that after Microcystis decomposition (day 14), the bacterial phylotype diversity in the sediment increased in enclosures with added Microcystis, and the relative abundance of bacteria affiliated with Bacteroidetes and Verrucomicrobia strongly increased. Canonical correspondence analysis showed that organic matter and Chl-a contributed significantly to the changes in sediment bacterial communities during Microcystis decomposition. The present results demonstrate Microcystis decomposition may directly and indirectly influence the BCC of the sediment.
在黑暗和缺氧条件下进行的为期 14 天的中观实验中，研究了微囊藻水华分解对沉积物细菌群落的影响，该实验中微囊藻生物量（用水柱中的叶绿素 a（Chl-a）浓度测量）有三个不同水平（从 20 到 2000μg/L）。由于水柱中 Chl-a 浓度的快速下降，在第 0 天和第 14 天采集沉积物样品以进行细菌群落组成（BCC）分析。BCC 通过变性梯度凝胶电泳和优势条带的测序来确定。结果表明，在微囊藻分解后（第 14 天），添加微囊藻的围隔中沉积物中的细菌种型多样性增加，与拟杆菌门和疣微菌门相关的细菌的相对丰度强烈增加。典范对应分析表明，有机质和 Chl-a 对微囊藻分解过程中沉积物细菌群落的变化有显著贡献。本研究结果表明，微囊藻分解可能直接和间接影响沉积物的 BCC。
