Division of Microbiology, Department of Botany, Faculty of Science, Sohag University, Sohag, Egypt.
J Basic Microbiol. 2013 Jun;53(6):477-88. doi: 10.1002/jobm.201100635. Epub 2012 Aug 23.
Twenty-three bacterial isolates from polluted water and soil were screened for heavy metals resistance (i.e., Al(3+), Co(2+), and Cu(2+)). The most potent isolate was identified by morphological characteristics, biochemical tests and confirmed by API20E kits as Providencia rettgeri MAM-4. Removal of Al(3+) from aqueous solution by P. rettgeri is more efficient (∼fourfold) than that by B. cereus ATCC 11778 (a comparison strain) at concentration of 200 mg L(-1) Al(3+). P. rettgeri was able to remove Co(2+) more than B. cereus ATCC 11778 at concentration of 50 mg L(-1) Co(2+). Inoculation of P. rettgeri into clay enhanced significantly the removal of Al(3+), Co(2+), and Cu(2+). P. rettegri MI (mutant strain) was able to tolerate more Al(3+) than that of the parent strain. P. rettgeri was resistant to 7 out of 15 antibiotics tested. P. rettgeri MAM-4 isolated from wastewater had ability to remove Al(3+), Co(2+), and Cu(2+) efficiently from aqueous media; and enhanced significantly metal biosporption by clay. This study has revealed that P. rettgeri could be employed as an effective and economic technology for the removal such metal elements from polluted environment.
从受污染的水和土壤中筛选出 23 株细菌分离株，以筛选其对重金属的抗性（即 Al(3+)、Co(2+)和 Cu(2+)）。通过形态特征、生化试验和 API20E 试剂盒鉴定，最有效的分离株被鉴定为 Providencia rettgeri MAM-4。与对照菌株 B. cereus ATCC 11778 相比，浓度为 200mg/L 的 Al(3+)时，P. rettgeri 从水溶液中去除 Al(3+)的效率更高（约四倍）。与对照菌株 B. cereus ATCC 11778 相比，浓度为 50mg/L 的 Co(2+)时，P. rettgeri 去除 Co(2+)的能力更强。将 P. rettgeri 接种到粘土中可显著增强对 Al(3+)、Co(2+)和 Cu(2+)的去除。P. rettegri MI（突变株）比亲株更能耐受 Al(3+)。P. rettgeri 对测试的 15 种抗生素中的 7 种具有抗性。从废水分离出的 P. rettgeri 能够有效地从水溶液中去除 Al(3+)、Co(2+)和 Cu(2+)；并显著增强了粘土对金属的生物吸附。这项研究表明，P. rettgeri 可以作为一种有效且经济的技术，用于从污染环境中去除这些金属元素。
