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长期电刺激对三维细胞培养物的影响：鸡胚脑球体

On the effect of long-term electrical stimulation on three-dimensional cell cultures: Hen embryo brain spheroids.

作者信息

Uroukov Ivan S, Bull Larry

机构信息

Faculty of Applied Sciences;

出版信息

Med Devices (Auckl). 2008 Jul;1:1-12. doi: 10.2147/mder.s3245. Epub 2008 Jul 8.

DOI:10.2147/mder.s3245
PMID:22915901
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3417903/
Abstract

A comprehensive dataset of multielectrode array recordings was collected from three-dimensional hen embryo brain cell cultures, termed spheroids, under long-term electrical stimulation. The aim is to understand the ongoing changes in the spiking activity under electrical stimulation within the lifetime of 14-72DIV of the neuronal networks contained therein. The spiking dynamics were analyzed and behavioral characteristics derived. Some effects on spiking patterns and exhaustion were followed in culture lifetime. With respect to the culture development, two main types of spiking exhaustion were found: one which materializes in the form of a drop in the sporadic (tonic) spiking frequency at the later maturation stages; and another associated with decreasing spiking train appearance throughout an experimental period.

摘要

从三维鸡胚脑细胞培养物（称为球体）中收集了一个多电极阵列记录的综合数据集，该培养物处于长期电刺激之下。目的是了解其中包含的神经元网络在14 - 72天体外培养期内电刺激下尖峰活动的持续变化。分析了尖峰动力学并得出行为特征。在培养期内跟踪了对尖峰模式和耗竭的一些影响。关于培养物的发育，发现了两种主要类型的尖峰耗竭：一种在后期成熟阶段以散发性（紧张性）尖峰频率下降的形式出现；另一种与整个实验期内尖峰序列出现减少有关。

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Neurosci Lett. 2006 Aug 14;404(1-2):33-8. doi: 10.1016/j.neulet.2006.05.016. Epub 2006 Jun 5.
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