Kohl S, Pickering L K, Diluzio N R
J Immunol Methods. 1979;29(4):361-8. doi: 10.1016/0022-1759(79)90007-3.
The effect of short term in vitro incubation of glucan--a reticuloendothelial system stimulator--on subsequent cytotoxicity of human monocyte-macrophages (MP) and lymphocytes (L) to Herpes simplex virus-infected cells in a 51Cr-release assay was analyzed. Particulate, cell-associated glucan irreversibly inhibited MP antibody-dependent cellular cytotoxicity (ADCC). In contrast, the inhibition of L-ADCC and L-natural killer cytotoxicity could be reversed by dissociation of glucan and cells utilizing serum gradient centrifugation, a process which did not remove the glucan. These experiments reveal further basic differences between MP and L-ADCC using the reagent glucan.
分析了葡聚糖（一种网状内皮系统刺激剂）短期体外孵育对人单核细胞 - 巨噬细胞（MP）和淋巴细胞（L）随后在51Cr释放试验中对单纯疱疹病毒感染细胞的细胞毒性的影响。颗粒状、与细胞相关的葡聚糖不可逆地抑制MP抗体依赖性细胞毒性（ADCC）。相反，利用血清梯度离心法分离葡聚糖和细胞可逆转对L - ADCC和L - 自然杀伤细胞毒性的抑制，该过程不会去除葡聚糖。这些实验揭示了使用试剂葡聚糖时MP和L - ADCC之间进一步的基本差异。
