Center for Nanoscale Materials, Argonne National Laboratory, Argonne, Illinois 60439, USA.
J Am Chem Soc. 2012 Sep 12;134(36):14646-9. doi: 10.1021/ja304674d. Epub 2012 Aug 28.
Long fibers assembled from peptide amphiphiles capable of binding the metalloporphyrin zinc protoporphyrin IX ((PPIX)Zn) have been synthesized. Rational peptide design was employed to generate a peptide, c16-AHL(3)K(3)-CO(2)H, capable of forming a β-sheet structure that propagates into larger fibrous structures. A porphyrin-binding site, a single histidine, was engineered into the peptide sequence in order to bind (PPIX)Zn to provide photophysical functionality. The resulting system indicates control from the molecular level to the macromolecular level with a high order of porphyrin organization. UV/visible and circular dichroism spectroscopies were employed to detail molecular organization, whereas electron microscopy and atomic force microscopy aided in macromolecular characterization. Preliminary picosecond transient absorption data are also reported. Reduced hemin, (PPIX)Fe(II), was also employed to highlight the material's versatility and tunability.
已合成了能够结合金属卟啉锌原卟啉 IX((PPIX)Zn)的肽两亲物的长纤维。合理的肽设计被用来生成一种肽，c16-AHL(3)K(3)-CO2H，它能够形成一个β-折叠结构，从而扩展成更大的纤维结构。一个卟啉结合位点，一个组氨酸，被设计到肽序列中，以结合(PPIX)Zn，从而提供光物理功能。所得系统表明，具有高卟啉组织有序性的从分子水平到高分子水平的控制。使用紫外/可见和圆二色性光谱详细描述分子组织，而电子显微镜和原子力显微镜有助于大分子表征。还报告了初步的皮秒瞬态吸收数据。还使用还原血红素，(PPIX)Fe(II)，来突出材料的多功能性和可调谐性。
