Mejía Omar, León-Romero Yatzil, Soto-Galera Eduardo
Laboratorio de Variación Biológica y Evolución, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas-IPN, Carpio esq. Plan de Ayala s/n Col. Casco de Santo Tomás, México DF C.P. 11340, Mexico.
Mitochondrial DNA. 2012 Dec;23(6):471-6. doi: 10.3109/19401736.2012.710207. Epub 2012 Aug 24.
The Pánuco-Tamesí complex in eastern Mexico is globally recognized as an important ecoregion due to its high level of endemism. In this study, DNA barcodes were generated for 152 individuals of 31 species. Additionally, 170 DNA barcodes for the related species available in the Barcode of Life Database (BOLD) system were included to test the ability of barcoding technique to discriminate between the closely related species. DNA barcoding allowed the discrimination of 79.2% of the analyzed species; poor resolution was observed in four genera in which the levels of resolution ranged from 16.6% in the genus Herichthys to 77.7% in the genus Xiphophorus. The results of this study demonstrate that DNA barcoding is a useful exploratory tool but fails to discriminate between closely related species.
墨西哥东部的帕努科 - 塔梅西复合体因其高度的特有性而被全球公认为一个重要的生态区。在本研究中，为31个物种的152个个体生成了DNA条形码。此外，还纳入了生命条形码数据库（BOLD）系统中相关物种的170个DNA条形码，以测试条形码技术区分近缘物种的能力。DNA条形码能够区分79.2%的分析物种；在四个属中观察到分辨率较差，其中分辨率水平从赫氏丽体鱼属的16.6%到剑尾鱼属的77.7%不等。本研究结果表明，DNA条形码是一种有用的探索工具，但无法区分近缘物种。
