Graduate School of Biological Science, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.
Plant Signal Behav. 2012 Nov;7(11):1388-92. doi: 10.4161/psb.21825. Epub 2012 Aug 23.
2-Hydroxy fatty acids mainly contained in sphingolipids are synthesized by a sphingolipid fatty acid 2-hydroxylase (FAH). Recently, two FAH homologs in Arabidopsis thaliana (AtFAH1 and AtFAH2), without any cytochrome b₅(Cb5)-like domains, which are essential for the function of Saccharomyces cerevisiae and mammalian FAH, were identified and both AtFAHs were shown to be activated by the interaction with Cb5. In this study, we compared FAHs of various plants, animals and fungi. Interestingly, only plants had two FAH homologs and none of plant FAHs had any Cb5-like domains. In addition, we showed from the interaction and expression analyses that AtFAHs interacted with multiple Cb5s probably in various tissues. Thus, plant FAHs may have evolved unlike animal and fungus FAHs.
2-羟脂肪酸主要存在于鞘脂中，由鞘脂脂肪酸 2-羟化酶（FAH）合成。最近，在拟南芥中鉴定出了两种 FAH 同源物（AtFAH1 和 AtFAH2），它们没有任何细胞色素 b₅（Cb5）样结构域，而后者对于酿酒酵母和哺乳动物 FAH 的功能至关重要，并且发现 AtFAHs 均通过与 Cb5 的相互作用而被激活。在本研究中，我们比较了各种植物、动物和真菌中的 FAHs。有趣的是，只有植物具有两个 FAH 同源物，并且植物 FAHs 均没有任何 Cb5 样结构域。此外，我们通过相互作用和表达分析表明，AtFAHs 可能在不同组织中与多种 Cb5 相互作用。因此，植物 FAHs 的进化可能与动物和真菌 FAHs 不同。
